Identification of genes differentially expressed in onion infected with Iris yellow spot virus

Ahmed ABDELKHALEK, Adel ELMORSI, Omar ALSHEHABY, Neeti SANAN-MISHRA, Elsayed HAFEZ

Abstract


Iris yellow spot virus (IYSV) causes severe damage and economic losses in onion production. Differential display-PCR was used to study changes in the gene expression of IYSV-infected onion plants. Representative up-regulated and down-regulated genes were selected for further study. Based on sequence analysis, the up-regulated genes were identified as retrotransposon protein, disease resistance-like proteins, chitinase, pathogenesis-related protein, cytochrome oxidase, cytochrome c, pentatricopeptide repeat-containing protein and pectin methylesterase. A DNA-binding transcriptional repressor protein gene was greatly down-regulated. . Most of the identified genes are known to play essential roles in plant defence systems, and are newly identified in onion sequences.


Keywords


DD-PCR; defence system; IYSV; up/down-regulated genes

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DOI: http://dx.doi.org/10.14601/Phytopathol_Mediterr-21877



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