Biochemical, physiological changes and antioxidant responses of cut gladiolus flower 'White Prosperity' induced by nitric oxide

Hashem Kazemzadeh-Beneh, Davood Samsampour, Saeedeh Zarbakhsh

Abstract


Sodium nitroprusside (SNP), as nitric oxide (NO) donor, has been considered by postharvest researchers as one of the best option for slowing the processes controlling senescence in cut flowers. Here, we investigate the role of NO on postharvest physiology and vase life of the Gladiolus grandiflorus cv. White Prosperity. Vase life markedly extended by SNP at 150 μM from 3 day to 7.33 day and thus those inducer effects were dose- and time-dependent. SNP at 125 μM interdependent on vase life time period was observed to be the optimal dose for improving of relative fresh weight (RFW), peroxidase (POD), and total monomeric anthocyanin (TMA) in cut flowers. Supplementing vase solution with SNP indicated significant increase in water uptake of cut flowers and consequently protected to decline in RFW due to alleviate water losses stress. SNP was maintained the level of total soluble protein, lipid peroxidation, and POD, whereas it enhanced the level of catalase (CAT) and TMA in flower petals. Summary of our results revealed that SNP exogenous prolongs vase life via maintaining protein degrade, scavenging free radical in term of anthocyanin and enzymes antioxidant, decreasing polyphenol oxidase, inhibiting lipid peroxidation, and improving membrane stability in ‘White Prosperity’ cut flowers.

Keywords


Anthocyanin; catalase; cut gladiolus flower; enzymatic antioxidant system; nitric oxide (NO); sodium nitroprusside (SNP)

Full Text:

PDF


DOI: http://dx.doi.org/10.13128/ahs-23361



This work is licensed under a Creative Commons Attribution 4.0 International License (CC-BY- 4.0)
 
Firenze University Press
Via Cittadella, 7 - 50144 Firenze
Tel. (0039) 055 2757700 Fax (0039) 055 2757712
E-mail: info@fupress.com