DNA changes in cotton (Gossypium hirsutum L.) under salt stress as revealed by RAPD marker

B. Saleh

Abstract


Random amplified polymorphic DNA (RAPD) analysis was applied to evaluate DNA changes among four upland cotton (Gossypium hirsutum L.) varieties [Niab 78 (N78), Deir-Ezzor 22 (DE22), Deltapine 50 (DP50) and Aleppo 118 (A118)] grown under non-saline conditions (control) and salt stress (200 mM NaCl) for seven weeks. Changes in RAPD profiles were measured as genomic template stability (GTS%). The highest estimated GTS% value was recorded for the two sensitive varieties, DP50 (79.1%) followed by A118 (58.2%); whereas, the lowest value was recorded for the two other tolerant varieties DE22 (36.7%) followed by N78 (26.4%). Based upon the data presented, RAPD marker could be used as potential tool for early identification of cotton tolerance to salt stress.

Keywords


cotton; genomic template stability; RAPD; salt stress

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DOI: http://dx.doi.org/10.13128/ahs-18697



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